You can substitute the Flank Steak with Eye of Round Fast-Fry Steak (a steak that is about ¼ inch/5 mm thick) and cut the steak into strips that are about 1 inch (2.5 cm) wide. While commercial jerky lasts at room temperature for a long time, homemade jerky is good for just a short time at room temperature and is best stored in the fridge or freezer for longer storage.